CTAB Precipitation Buffer

CTAB Precipitation Buffer

Purpose:

  • Selectively precipitates DNA–CTAB complexes, while many polysaccharides remain soluble.
  • Especially important for plants rich in polysaccharides, mucilage, or secondary metabolites.
  • Improves purity compared to a direct alcohol precipitation.

Components: Typically 1–2% CTAB in high-salt solution.

  • CTAB: detergent that lyses cells and binds polysaccharides.
  • Tris-HCl: maintains stable pH.
  • EDTA: chelates Mg²⁺, inhibiting DNases.
  • NaCl: promotes dissociation of polysaccharides and proteins from DNA.

 

Ingredient

Concentration

CTAB

1%

Tris

50mM

EDTA

10 mM

NaCL

40 mM

 

Preparation

CTAB Precipitation Buffer

Recipe calculator · Scales ingredients to target volume
Step 1. Prepare ingredients.
10% CTAB STOCK
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Step 2. Prepare ingredients.
1M Tris Stock (pH 8)
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Step 3. Prepare ingredients.
0.5M EDTA STOCK
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Step 4. Prepare ingredients.
5M NaCl Stock
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Step 5. Bring final volume to 50 mL with water.