- 0.5M EDTA STOCK
- 0.9% Saline Solution
- 10% Chelex Solution
- 10% CTAB STOCK
- 10% SDS
- 1M DTT
- 1M Glucose
- 1M NaOH
- 1M Tris (pH 8)
- 5M NaCl Stock
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5M Potassium acetate
Add 40 mL of distilled water to a 100ml beaker. Gradually add 24.5 g of potassium acetate while stirring until fully dis...
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5M Potassium acetate
Add 40 mL of distilled water to a 100ml beaker. Gradually add 24.5 g of potassium acetate while stirring until fully dis...
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CTAB Lysis Buffer
CTAB Extraction Buffer Components: CTAB (cetyltrimethylammonium bromide), Tris-HCl, EDTA, NaCl, β-mercaptoethanol, and P...
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CTAB Precipitation Buffer
CTAB Precipitation Buffer Purpose: Selectively precipitates DNA–CTAB complexes, while many polysaccharides remain solubl...
- Digestion Buffer, DTT extraction
- Lysis buffer I
- Plasmid Miniprep Solution I - Resuspension Buffer
- Plasmid Miniprep Solution II - Denaturing Solution
- Resuspension Buffer (Alkaline Lysis Plasmid Miniprep)
- 1% Agarose Gel
- 1x Lithium Acetate Borate (LAB) Buffer
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25x Lithium Acetate Borate (LAB) Buffer
To make a 1x working solution, dilute 40 mL of 25X stock solution with 960 mL deionized H₂O. Mix well.
- Artificial Seawater (ASW)
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ATCC Medium 260: Tryptic Soy Medium with 5% Defibrinated Sheep Blood
Agar MediumTryptic Soy Agar (BD 236950) ……………….… 40.0 gDI Water……………………………………….…. 950.0 mLBroth MediumTryptic Soy Broth...
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ATCC Medium 2722: Supplemented Tryptic Soy Broth
ATCC Medium 2722: Supplemented Tryptic Soy BrothTryptic Soy Broth…………………………………………… 30.0 gYeast Extract………………………………………………...
- Chloramphenicol Stock
- LB Agar Plates
- Seawater Tryptone Plates
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