1x Seawater Salt Solution for Serial Dilution
Preparation
1x Seawater Salt Solution for Serial Dilution
Step 1.
Measure into media bottle.
Simplified Seawater Medium for Marine Bacteria, Stock A (10x)
100
mL
Simplified Seawater Medium for Marine Bacteria, Stock B (10x)
100
mL
Step 2.
Bring final volume to 1000 mL with distilled water.
Nested recipe inputs
Used in step 1
Simplified Seawater Medium for Marine Bacteria, Stock A (10x)
Step 1.
Weigh into appropriately-sized flask.
Sodium Chloride
211.9
g
Sodium Sulfate, Anhydrous
35.5
g
Potassium Chloride
6
g
Step 2.
Add approximately 700 mL distilled water. Stir on heated stirrer until salts dissolve.
Step 3.
Bring final volume to 1000 mL with distilled water.
Step 4.
Autoclave at 121 °C for 20 min (liquid cycle).
Used in step 1
Simplified Seawater Medium for Marine Bacteria, Stock B (10x)
Step 1.
Weigh ingredients into appropriately-sized flask.
Magnesium Chloride Hexahydrate
95.9
g
Calcium Chloride Dihydrate
13.44
g
Step 2.
Add approximately 700 mL distilled water. Stir on heated stirrer until salts dissolve.
Step 3.
Bring final volume to 1000 mL with distilled water.
Step 4.
Autoclave at 121 °C for 20 min (liquid cycle).