PCR calculator

Master mix volumes for any reaction size and number of reactions

Master mix

Different templates, same primers: primers go in the mix, template goes into each tube · water makes up the rest · master mix includes overage for pipetting loss
Master mix
Per reaction
Total (1× + 10%)
Buffer (10×)
2.5 µL
2.75 µL
dNTPs
0.5 µL
0.55 µL
MgCl₂
1.5 µL
1.65 µL
Forward primer
0.5 µL
0.55 µL
Reverse primer
0.5 µL
0.55 µL
Taq polymerase
0.1 µL
0.11 µL
Nuclease-free water
18.4 µL
20.24 µL
Master mix total
24 µL
26.4 µL
Add to each tube
Per reaction
Master mix
24 µL
Template DNA
1 µL
Each tube holds 25 µL: 24 µL of master mix plus 1 µL Template DNA.