Simplified Seawater Medium for Marine Bacteria, Stock B (10x)

Preparation

Target volume

Unit

Step 1. Weigh ingredients into appropriately-sized flask.

Magnesium Chloride Hexahydrate

95.9 g

Calcium Chloride Dihydrate

13.44 g

Step 2. Add approximately 700 mL distilled water. Stir on heated stirrer until salts dissolve.

Step 3. Bring final volume to 1000 mL with distilled water.

Step 4. Autoclave at 121 °C for 20 min (liquid cycle).