Simplified Seawater Medium for Marine Bacteria, Stock A (10x)

Preparation

Target volume

Unit

Step 1. Weigh into appropriately-sized flask.

Sodium Chloride

211.9 g

Sodium Sulfate, Anhydrous

35.5 g

Potassium Chloride

6 g

Step 2. Add approximately 700 mL distilled water. Stir on heated stirrer until salts dissolve.

Step 3. Bring final volume to 1000 mL with distilled water.

Step 4. Autoclave at 121 °C for 20 min (liquid cycle).