Artificial Sea Water Salts Broth

Recommended for culturing marine bacteria.

Preparation

Target volume

Unit

Step 1. Weigh all ingredients into a 2L flask

Sodium Chloride

24.6 g

Potassium Chloride

0.67 g

Calcium Chloride Dihydrate

1.36 g

Magnesium Sulfate, Heptahydrate

6.29 g

Magnesium Chloride Hexahydrate

4.66 g

Sodium Bicarbonate

0.18 g

Step 2. Bring final volume to 1000 mL with distilled water.