Recipes
Simplified Seawater Agar Medium for Marine Bacteria

Simplified Seawater Agar Medium for Marine Bacteria

Preparation

Target volume

Unit

Step 1. Add to flask

Simplified Seawater Medium for Marine Bacteria, Stock A (10x)

100 mL

Peptone

5 g

Yeast Extract

1 g

Agar

15 g

Dipotassium phosphate (K₂HPO₄), 1M

100 μL

Step 2. Add approximately 800 mL distilled water. Adjust pH to 7.6 with dilute Sodium Hydroxide or Hydrochloric Acid

Step 3. Bring volume to 899 mL with distilled water (1000 mL final, less 101 mL added in later steps).

Step 4. Autoclave at 121 °C for 20 min (liquid cycle).

Step 5. Bring autoclaved stock to 50–55 °C, then add

Simplified Seawater Medium for Marine Bacteria, Stock B (10x) sterile

100 mL

Simplified Seawater Medium for Marine Bacteria, Stock C (1000x) sterile

1 mL

Nested recipe inputs

Used in step 1

Target volume

Unit

Step 1. Weigh into appropriately-sized flask.

Sodium Chloride

211.9 g

Sodium Sulfate, Anhydrous

35.5 g

Potassium Chloride

6 g

Step 2. Add approximately 700 mL distilled water. Stir on heated stirrer until salts dissolve.

Step 3. Bring final volume to 1000 mL with distilled water.

Step 4. Autoclave at 121 °C for 20 min (liquid cycle).

Used in step 1

Target volume

Unit

Step 1. Measure into appropriately-sized flask.

Potassium Phosphate, Dibasic

8.71 g

Step 2. Filter-sterilize through a 0.22 µm filter.

Step 3. Bring final volume to 50 mL with distilled water.

Used in step 5

Target volume

Unit

Step 1. Weigh ingredients into appropriately-sized flask.

Magnesium Chloride Hexahydrate

95.9 g

Calcium Chloride Dihydrate

13.44 g

Step 2. Add approximately 700 mL distilled water. Stir on heated stirrer until salts dissolve.

Step 3. Bring final volume to 1000 mL with distilled water.

Step 4. Autoclave at 121 °C for 20 min (liquid cycle).

Used in step 5

Target volume

Unit

Step 1. Weigh into appropriately-sized container.

Ferrous Sulfate Heptahydrate

0.278 g

Sodium Citrate

0.59 g

Step 2. Bring final volume to 100 mL with distilled water.

Step 3. Filter-sterilize through a 0.22 µm filter.