1% Agarose Gel
Main Ingredients
| Ingredient | Amount |
|---|---|
| Agarose | 750 mg |
|
Agarose:
Add to a 250ml flask |
|
| 1x Lithium Acetate Borate (LAB) Buffer | 75 mL |
|
1x Lithium Acetate Borate (LAB) Buffer:
Pour into the flask then microwave for 1 minute and 30 seconds, until the agarose completely melted. |
|
| SYBR Safe DNA Gel Stain | 7.5 µL |
|
SYBR Safe DNA Gel Stain:
Add after microwaving and swirl. |
|
Stock Solution: 1x Lithium Acetate Borate (LAB) Buffer
| Ingredient | Amount |
|---|---|
| 25x Lithium Acetate Borate (LAB) Buffer | 40 mL |
| Bring to 1 L with water | |
Stock Solution: 25x Lithium Acetate Borate (LAB) Buffer
| Ingredient | Amount |
|---|---|
| Lithium Acetate Dihydrate | 25.5 g |
| Boric Acid | 15.5 g |
| Bring to 1 L with water | |