Resuspension Buffer (Alkaline Lysis Plasmid Miniprep)
Preparation
Resuspension Buffer (Alkaline Lysis Plasmid Miniprep)
Step 1.
Prepare ingredients.
0.5M EDTA STOCK
—
Step 2.
Prepare ingredients.
1M Tris (pH 8)
—
Step 3.
Prepare ingredients.
1M Glucose
—
Step 4.
Bring final volume to 100 mL with distilled water.
Nested recipe inputs
Used in step 1
0.5M EDTA STOCK
Step 1.
Prepare ingredients.
EDTA
7.306
g
(0.5 M)
Step 2.
Bring final volume to 50 mL with distilled water.
Used in step 2
1M Tris (pH 8)
Step 1.
Add to appropriate container.
Tris Base
12.114
g
Step 2.
Bring final volume to 100 mL with distilled water. Fill media bottle to with ~80% target volume with distilled water.
Step 3.
Add a magnetic stirrer and place on a stirring plate to mix the solution.
Step 4.
Use a pH meter to measure pH. Slowly add concentrated hydrochloric acid (HCl) solution using a Pasteur pipette to reduce the pH to 8.0.
Be careful not to add too much at a time, since the pH will change rapidly.
Step 5.
Bring final volume to 100 mL with distilled water. Once the desired pH has been reached, bring to final volume using distilled water.
Used in step 3
1M Glucose
Step 1.
Prepare ingredients.
D-Glucose Anhydrous
9.009
g
Step 2.
Bring final volume to 50 mL with distilled water.