1M Tris (pH 8)

Preparation

Target volume

Unit

Step 1. Weigh out the Tris and add to a media bottle.

Tris Base

12.114 g

Step 2. Fill media bottle to with ~80% target volume with distilled water.

Step 3. Add a magnetic stirrer and place on a stirring plate to mix the solution.

Step 4. Use a pH meter to measure pH. Slowly add concentrated hydrochloric acid (HCl) solution using a Pasteur pipette to reduce the pH to 8.0.

Be careful not to add too much at a time, since the pH will change rapidly.

Step 5. Once the desired pH has been reached, bring to final volume using distilled water.

Step 6. Bring final volume to 100 mL with water.