1M Tris (pH 8)

Preparation

Target volume

Unit

Step 1. Add to appropriate container.

Tris Base

12.114 g

Step 2. Bring final volume to 100 mL with distilled water. Fill media bottle to with ~80% target volume with distilled water.

Step 3. Add a magnetic stirrer and place on a stirring plate to mix the solution.

Step 4. Use a pH meter to measure pH. Slowly add concentrated hydrochloric acid (HCl) solution using a Pasteur pipette to reduce the pH to 8.0.

Be careful not to add too much at a time, since the pH will change rapidly.

Step 5. Bring final volume to 100 mL with distilled water. Once the desired pH has been reached, bring to final volume using distilled water.