Wash Buffer

Preparation

Wash Buffer

Recipe calculator · Scales ingredients to target volume
Step 1. Add to appropriate container.
Ethanol, 200 proof
80 mL
5M NaCl Stock
400 μL
Tris, 1M, pH 7.5
200 μL
Step 2. Bring final volume to 100 mL with distilled water.

Nested recipe inputs

Used in step 1

5M NaCl Stock

Recipe calculator · Scales ingredients to target volume
Step 1. Prepare ingredients.
Sodium Chloride
14.61 g (5 M)
Step 2. Bring final volume to 50 mL with distilled water.
Used in step 1

Tris, 1M, pH 7.5

Recipe calculator · Prepares solution at target volume
Step 1. Add approximately 35 mL distilled water.
Step 2. Dissolve.
Tris Base
12.114 g
Step 3. Adjust to pH 7.5 with concentrated HCl (~12 M). Expect to need roughly 6–7 mL.
Step 4. Bring final volume to 50 mL with distilled water.
Step 5. Autoclave at 121 °C for 20 min (liquid cycle).