Hemin Stock Solution (5 mg/mL)
Preparation
Hemin Stock Solution (5 mg/mL)
Step 1.
Add to beaker
Hemin
0.5
g
Step 2.
Add NaOH. Stir or vortex vigorously until the powder dissolves completely into a dark, opaque fluid. Ensure no visible particles remain on the glass walls.
1M NaOH
10
mL
Step 3.
Bring to final volume using \(dH_{2}O\).
Step 4.
Autoclave the solution at 121°C for 15 minutes. (Hemin is heat-stable).
Store the sterilized solution in an amber glass bottle at 4°C.
Add 1 mL of this stock solution per 1 Liter of media to achieve a final concentration of 5 mg/L
Nested recipe inputs
Used in step 2
1M NaOH
Work in chemical hood and wear eye protection. NaOH is extermely caustic!
Step 1.
Add ~75% of the target volume in distilled water to a appropriately-sized beaker.
Step 2.
Gradually add NaOH pellets while stirring.
Sodium Hydroxide
2
g
The solution will heat up. This is normal.
Step 3.
After fully dissolved, transfer the solution to a falcon tube or media bottle.
Step 4.
Bring final volume to 50 mL with distilled water.