Plasmid Miniprep Solution II - Denaturing Solution

Preparation

Plasmid Miniprep Solution II - Denaturing Solution

Recipe calculator · Scales ingredients to target volume
Step 1. Prepare ingredients.
1M NaOH
10 mL
Step 2. Prepare ingredients.
10% SDS
5 mL

Store at room temperature.

Step 3. Bring final volume to 50 mL with water.

Nested recipe inputs

Used in step 1

1M NaOH

Recipe calculator · Scales ingredients to target volume

Work in chemical hood and wear eye protection. NaOH is extermely caustic!

Step 1. Add ~75% of the target volume in distilled water to a appropriately-sized beaker.
Step 2. Gradually add NaOH pellets while stirring.
Sodium Hydroxide
2 g

The solution will heat up. This is normal.

Step 3. After fully dissolved, transfer the solution to a falcon tube or media bottle.
Step 4. Bring final volume to 50 mL with water.
Used in step 2

10% SDS

Recipe calculator · Scales ingredients to target volume
Step 1. Prepare ingredients.
Sodium Lauryl Sulfate
5 g
Step 2. Bring final volume to 50 mL with water.