Binding Buffer

Preparation

Binding Buffer

Recipe calculator · Scales ingredients to target volume
Step 1. Add approximately 35 mL distilled water.
Step 2. Add to water
Guanidine Hydrochloride
47.8 g
Sodium Acetate, 3M, pH 5.2
1 mL
Step 3. Dissolve Gu-HCL.
Step 4. Add to container
Isopropanol
30 mL
Step 5. Bring final volume to 100 mL with distilled water.

Nested recipe inputs

Used in step 2

Sodium Acetate, 3M, pH 5.2

Recipe calculator · Scales ingredients to target volume
Step 1. Weigh into appropriate container.
Sodium Acetate, Trihydrate
40.8 g
Step 2. Add approximately 60 mL distilled water.
Step 3. Adjust to pH 5.2 with glacial acetic acid. Expect to need roughly 6–8 mL
Step 4. Bring final volume to 100 mL with distilled water.
Step 5. Filter-sterilize through a 0.22 µm filter.